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验证数据展示

  • IF Staining of HeLa using 13554-1-AP

    IF Staining of HeLa using 13554-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13554-1-AP (HELIOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lymphoma using 13554-1-AP

    IHC staining of human lymphoma using 13554-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (HELIOS antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lymphoma using 13554-1-AP

    IHC staining of human lymphoma using 13554-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (IKZF2 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of Jurkat using 13554-1-AP

    WB analysis of Jurkat using 13554-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13554-1-AP (HELIOS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13554-1-AP

    IP experiment of Jurkat using 13554-1-AP

    IP result of anti-HELIOS (IP:13554-1-AP, 4ug; Detection:13554-1-AP 1:300) with Jurkat cells lysate 2400ug.

HELIOS Polyclonal antibody

HELIOS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13554-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

IKZF2, ZNFN1A2, ZNF1A2, Zinc finger protein Helios, IKZF 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, human placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13554-1-AP targets HELIOS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 HELIOS fusion protein Ag4357 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IKAROS family zinc finger 2 (Helios)
别名 IKZF2, ZNFN1A2, ZNF1A2, Zinc finger protein Helios, IKZF 2
计算分子量 500 aa, 57 kDa
观测分子量 65 kDa
GenBank蛋白编号BC028936
基因名称 HELIOS
Gene ID (NCBI) 22807
RRIDAB_2124959
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKS7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ikaros DNA-binding proteins, characterized by two highly conserved zinc finger domains that mediate DNA (N-terminal domain) and protein binding (C-terminal domain), consists of 5 members and one of them is IKZF2 [PMID:1439790]. IKZF2's expression is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685).

实验方案

Product Specific Protocols
WB protocol for HELIOS antibody 13554-1-APDownload protocol
IHC protocol for HELIOS antibody 13554-1-APDownload protocol
IF protocol for HELIOS antibody 13554-1-APDownload protocol
IP protocol for HELIOS antibody 13554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Immunohorizons

Clonal Bifurcation of Foxp3 Expression Visualized in Thymocytes and T Cells.

Authors - Bonnie Yen
View Article