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Aiolos/IKZF3 Polyclonal antibody, PBS Only

Aiolos/IKZF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19055-1-PBS

Print datasheet

Synonyms

IKZF3, ZNFN1A3, Zinc finger protein Aiolos, IKZF 3, AIOLOS

验证数据展示

  • WB analysis of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    WB analysis of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IKZF3 antibody included
  • IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IKZF3 antibody included
  • IF Staining of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    IF Staining of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody 19055-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IKZF3 Ready-To-Use IHC Kit. IKZF3 primary antibody included.

产品信息

19055-1-PBS targets Aiolos/IKZF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Aiolos/IKZF3 fusion protein Ag5537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IKAROS family zinc finger 3 (Aiolos)
Synonyms IKZF3, ZNFN1A3, Zinc finger protein Aiolos, IKZF 3, AIOLOS
Calculated Molecular Weight 509 aa, 58 kDa
Observed Molecular Weight 58-70 kDa
GenBank Accession NumberBC032707
Gene Symbol IKZF3
Gene ID (NCBI) 22806
RRIDAB_10643381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKT9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.