IL-1 beta Recombinant antibody

IL-1 beta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82696-15-RR

产品说明书

CloneNo. 240902D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLPS and Brefeldin A treated THP-1 cells, A431 cells, HT-1080 cells, U-87 MG cells
Positive IF/ICC detected inLPS and Brefeldin A treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82696-15-RR targets IL-1 beta in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 1, beta
别名 IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin
计算分子量 269 aa, 31 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC008678
基因名称 IL1B
Gene ID (NCBI) 3553
ENSEMBL Gene IDENSG00000125538
RRIDAB_3670529
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01584
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

实验方案

Product Specific Protocols
WB protocol for IL-1 beta antibody 82696-15-RRDownload protocol
IF protocol for IL-1 beta antibody 82696-15-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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