IL-10 Polyclonal antibody
IL-10 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (5)
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
114
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein, ag14644 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20850-1-AP targets IL-10 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC, IF, ELISA |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, monkey, bovine |
| Immunogen | IL-10 fusion protein Ag14644 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interleukin 10 |
| Synonyms | CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF |
| Calculated Molecular Weight | 21 kDa |
| GenBank Accession Number | BC104252 |
| Gene Symbol | IL-10 |
| Gene ID (NCBI) | 3586 |
| RRID | AB_2878752 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22301 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-10 antibody 20850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure | ||
Nat Commun B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma. | ||
Microbiome Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice. | ||
Acta Biomater A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon. | ||
Acta Biomater Bio-inspired Glycosylated Nano-hydroxyapatites Enhance Endogenous Bone Regeneration by Modulating Macrophage M2 Polarization | ||
Int J Biol Macromol In situ polyphenol-adhesive hydrogel enhanced the noncarcinogenic repairing of KGF on the gut epithelial barrier on TNBS-induced colitis rats |
