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验证数据展示

  • WB analysis of Recombinant protein using 60269-1-Ig (same clone as 60269-1-PBS)

    WB analysis of Recombinant protein using 60269-1-Ig (same clone as 60269-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60269-1-Ig (IL-10 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

    IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60269-1-Ig (IL-10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

    IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60269-1-Ig (IL-10 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

  • IF Staining of MOLT-4 using 60269-1-Ig (same clone as 60269-1-PBS)

    IF Staining of MOLT-4 using 60269-1-Ig (same clone as 60269-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using IL-10 antibody (60269-1-Ig, Clone: 8E4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IL-10 Monoclonal antibody, PBS Only

IL-10 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60269-1-PBS

产品说明书

CloneNo. 8E4B6

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60269-1-PBS targets IL-10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 IL-10 fusion protein Ag14870 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 10
别名 CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF
计算分子量 21 kDa
观测分子量 22 kDa
GenBank蛋白编号BC104252
基因名称 IL-10
Gene ID (NCBI) 3586
RRIDAB_2881389
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP22301
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.