IL-10RA Recombinant antibody

IL-10RA Recombinant Antibody for WB, ELISA
Cat No. 84428-3-RR
CloneNo. 241833D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

IL10RA, IL 10R subunit alpha, IL 10R subunit 1, IL 10 receptor subunit alpha, HIL 10R

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84428-3-RR targets IL-10RA in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 10 receptor, alpha
Synonyms IL10RA, IL 10R subunit alpha, IL 10R subunit 1, IL 10 receptor subunit alpha, HIL 10R
Calculated Molecular Weight 578 aa, 63 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC028082
Gene Symbol IL-10RA
Gene ID (NCBI) 3587
RRIDAB_3671955
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RA, also known as IL-10R1 or CD210, is expressed on most hematopoietic cells at a basal level but is upregulated by various cells upon activation (PMID: 24507158). IL-10RA serves as the ligand-binding subunit of the receptor complex. Upon binding of IL-10 to the IL-10R complex, the kinases JAK1 and TYK2 are activated and catalyze the phosphorylation of IL-10RA at specific intracellular tyrosine residues, leading to the recruitment and subsequent phosphorylation of STAT3 (PMID: 11244051; 10433356).

实验方案

Product Specific Protocols
WB protocol for IL-10RA antibody 84428-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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