验证数据展示
经过测试的应用
| Positive WB detected in | Saos-2 supernatant cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68135-1-Ig targets IL-11 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | IL-11 fusion protein Ag11199 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 亲和力 | KD=1.80 x 10-9M KOff=2.20 x 10-6M KOn=1.22 x 103M |
| 全称 | interleukin 11 |
| 别名 | IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor |
| 计算分子量 | 199 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC012506 |
| 基因名称 | IL-11 |
| Gene ID (NCBI) | 3589 |
| RRID | AB_2923662 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20809 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-11 antibody 68135-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |