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验证数据展示

  • FC experiment of RAW 264.7 using 98004-1-PBS

    FC experiment of RAW 264.7 using 98004-1-PBS

    1X10^6 unstimulated or IFNγ and LPS treated (in the presence of protein transport inhibitors) RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-12/23 p40 (98004-1-PBS, Clone: 230037C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:700. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of RAW 264.7 using 98004-1-RR (same clone as 98004-1-PBS)

    FC experiment of RAW 264.7 using 98004-1-RR (same clone as 98004-1-PBS)

    1X10^6 unstimulated or IFNγ and LPS treated (in the presence of protein transport inhibitors) RAW 264.7 cells were intracellularly stained with 0.125 ug Anti-Mouse IL-12/IL-23 p40 (98004-1-RR, Clone: 230037C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:700. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98004-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98004-1-RR

    Affinity and Kinetic Characterization of 98004-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98004-1-RR against Mouse IL-12/23 p40 were performed. The affinity constant is 4.18 nM.

Anti-Mouse IL-12/IL-23 p40 Rabbit Recombinant Antibody, PBS Only

IL-12/IL-23 p40 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98004-1-PBS

产品说明书

CloneNo. 230037C9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inIFNγ and LPS treated RAW 264.7 cells

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98004-1-PBS targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IL-12/IL-23 p40
别名 Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b
计算分子量70 kDa
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP43432
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

实验方案

Product Specific Protocols
FC protocol for IL-12/IL-23 p40 antibody 98004-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols