验证数据展示
经过测试的应用
Positive IF/ICC detected in | MOLT-4 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83008-7-RR targets IL-12/IL-23 p40 in IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) |
别名 | IL12B, IL 12B, IL 12 subunit p40, IL 12 P40, Cytotoxic lymphocyte maturation factor 40 kDa subunit |
计算分子量 | 328 aa, 37 kDa |
GenBank蛋白编号 | BC067498 |
基因名称 | IL12B |
Gene ID (NCBI) | 3593 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P29460 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).
实验方案
Product Specific Protocols | |
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IF protocol for IL-12/IL-23 p40 antibody 83008-7-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |