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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 82878-2-RR

    WB analysis using 82878-2-RR

    Recombinant protein Eg0742 (IL-12 p70) and Recombinant protein Eg1821 (IL-12A p35)were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82878-2-RR

    Affinity and Kinetic Characterization of 82878-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82878-2-RR against Mouse IL-12 p70 were performed. The affinity constant is 7.37 nM.

IL-12 p70 Recombinant antibody

IL-12 p70 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82878-2-RR

产品说明书

CloneNo. 230037B7

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, ELISA

别名

Il 12b, Il 12p40, IL-12 P35, IL-12 p70, Il12b, Il12p40, interleukin 12b, p40

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82878-2-RR targets IL-12 p70 in WB, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IL-12 p70
别名 Il 12b, Il 12p40, IL-12 P35, IL-12 p70, Il12b, Il12p40, interleukin 12b, p40
计算分子量70 kDa
观测分子量70 kDa
基因名称
Gene ID (NCBI)
RRIDAB_3670597
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP43432(IL12B);P43431(IL12A)
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.

实验方案

Product Specific Protocols
WB protocol for IL-12 p70 antibody 82878-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols