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验证数据展示

  • WB analysis using 30956-1-AP

    WB analysis using 30956-1-AP

    Human IL-12B/p40, IL-12/p70, IL-12A/p35 recombinant protein, were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 30956-1-AP

    FC experiment of Jurkat using 30956-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IL-12/IL-23 p40 Polyclonal antibody

IL-12/IL-23 p40 Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 30956-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

IL12B, IL 23, IL 12B, IL 12 subunit p40, IL 12 P40

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein, HepG2 cells, Jurkat cells
Positive FC (Intra) detected inJurkat cells, MOLT-4 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30956-1-AP targets IL-12/IL-23 p40 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
别名 IL12B, IL 23, IL 12B, IL 12 subunit p40, IL 12 P40
计算分子量 328 aa, 37 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC067498
基因名称 IL12B
Gene ID (NCBI) 3593
RRIDAB_3669795
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29460
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

实验方案

Product Specific Protocols
WB protocol for IL-12/IL-23 p40 antibody 30956-1-APDownload protocol
FC protocol for IL-12/IL-23 p40 antibody 30956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols