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验证数据展示

  • WB analysis of K-562 using 13287-1-AP (same clone as 13287-1-PBS)

    WB analysis of K-562 using 13287-1-AP (same clone as 13287-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13287-1-AP (IL12RB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13287-1-AP (same clone as 13287-1-PBS)

    IHC staining of human tonsillitis using 13287-1-AP (same clone as 13287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13287-1-AP (IL12RB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 13287-1-AP (same clone as 13287-1-PBS)

    WB analysis of Jurkat using 13287-1-AP (same clone as 13287-1-PBS)

    WB result of IL-12RB1 antibody (13287-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells. This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 13287-1-AP (same clone as 13287-1-PBS)

    IP experiment of K-562 using 13287-1-AP (same clone as 13287-1-PBS)

    IP result of anti-IL-12RB1 (IP:13287-1-AP, 4ug; Detection:13287-1-AP 1:300) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IL-12RB1 Polyclonal antibody, PBS Only

IL-12RB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13287-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13287-1-PBS targets IL-12RB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 IL-12RB1 fusion protein Ag3983 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 12 receptor, beta 1
别名
计算分子量 662 aa, 73 kDa
观测分子量 100 kDa
GenBank蛋白编号BC029121
基因名称 IL12RB1
Gene ID (NCBI) 3594
RRIDAB_2124039
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42701
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.