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IL-13Ra1 Polyclonal antibody

IL-13Ra1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27916-1-AP

Print datasheet

Synonyms

IL13RA1, IL 13R subunit alpha 1, IL 13R alpha 1, IL 13 receptor subunit alpha 1, CT19

验证数据展示

  • WB analysis using 27916-1-AP

    WB analysis using 27916-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27916-1-AP (IL-13RA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHT-29 cells, Jurkat cells, Raji cells, mouse skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27916-1-AP targets IL-13Ra1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen IL-13Ra1 fusion protein Ag25542 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 13 receptor, alpha 1
Synonyms IL13RA1, IL 13R subunit alpha 1, IL 13R alpha 1, IL 13 receptor subunit alpha 1, CT19
Calculated Molecular Weight 49 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC009960
Gene Symbol IL-13Rα1
Gene ID (NCBI) 3597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78552
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a 65- to 70-kd glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).

实验方案

Product Specific Protocols
WB protocol for IL-13Ra1 antibody 27916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols