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  • Featured Product
  • KD/KO Validated

IL-17A Monoclonal antibody, PBS Only

IL-17A Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D5

Cat no : 66148-1-PBS

Print datasheet

Synonyms

IL 17, IL17, IL-17, IL17A, IL 17A

验证数据展示

  • WB analysis using 66148-1-Ig (same clone as 66148-1-PBS)

    WB analysis using 66148-1-Ig (same clone as 66148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

    IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

产品信息

66148-1-PBS targets IL-17A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen IL-17A fusion protein Ag3733 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 17A
Synonyms IL 17, IL17, IL-17, IL17A, IL 17A
Calculated Molecular Weight 155 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC067505
Gene Symbol IL-17A
Gene ID (NCBI) 3605
ENSEMBL Gene IDENSG00000112115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16552
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.