验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA and ionomycin stimulated C57BL/6 Th17-polarized splenocytes |
| Positive FC detected in | PMA and ionomycin stimulated C57BL/6 Th17-polarized splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98005-1-PBS targets IL-17A in FC (Intra) applications and shows reactivity with Mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | interleukin 17A |
| 别名 | Il17a, IL-17A, interleukin 17A, Interleukin17A, 230038C7, IL 17A, Il17 |
| 计算分子量 | 17 kDa |
| GenBank蛋白编号 | NM-010552 |
| 基因名称 | Il17a |
| Gene ID (NCBI) | 16171 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q62386 |
| 储存缓冲液 | PBS only , pH 7.3 |
| 储存条件 | Store at -80°C. Stable for one year after shipment. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-17A antibody 98005-1-PBS | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |