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IL-2 Monoclonal antibody, PBS Only

IL-2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11C5

Cat no : 60306-1-PBS

Print datasheet

Synonyms

IL2, TCGF, Interleukin-2, interleukin 2, IL 2

验证数据展示

  • WB analysis using 60306-1-Ig (same clone as 60306-1-PBS)

    WB analysis using 60306-1-Ig (same clone as 60306-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60306-1-Ig (IL-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    IHC staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60306-1-Ig (IL2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    IF Staining of human tonsillitis using 60306-1-Ig (same clone as 60306-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using IL-2 antibody (60306-1-Ig, Clone: 2F11C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60306-1-PBS in a different storage buffer formulation.

产品信息

60306-1-PBS targets IL-2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen IL-2 fusion protein Ag10199 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interleukin 2
Synonyms IL2, TCGF, Interleukin-2, interleukin 2, IL 2
Calculated Molecular Weight 153 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC070338
Gene Symbol IL2
Gene ID (NCBI) 3558
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60568
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.