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Mouse IL-2 Recombinant antibody, PBS Only (Detector)

IL-2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240358B1

Cat no : 83547-3-PBS

Print datasheet

Synonyms

Il2, Interleukin-2, interleukin 2, Il 2, 240358B1

验证数据展示

  • WB analysis of EL-4 using 83547-3-RR (same clone as 83547-3-PBS)

    WB analysis of EL-4 using 83547-3-RR (same clone as 83547-3-PBS)

    Untreated, PMA, Ionomycin and Brefeldin A treated EL-4 cells were subjected to SDS PAGE followed by western blot with 83547-3-RR (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83547-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00533-2

    Cytometric bead array standard curve of MP00533-2

    Cytometric bead array standard curve of MP00533-2, MOUSE IL-2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83547-2-PBS. Detection antibody: 83547-3-PBS. Standard: Eg0762. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83547-3-RR

    Affinity and Kinetic Characterization of 83547-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83547-3-RR against Mouse IL-2 were performed. The affinity constant is 36.5 nM.

产品信息

83547-3-PBS targets IL-2 as part of a matched antibody pair:

MP00533-2: 83547-2-PBS capture and 83547-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2
Synonyms Il2, Interleukin-2, interleukin 2, Il 2, 240358B1
Calculated Molecular Weight 19 kDa
Observed Molecular Weight15 kDa
GenBank Accession Number NM_008366
Gene Symbol IL-2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04351
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-2 (Il2) is a protein initially identified as a T cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that Il2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R). Il2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.