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Anti-Rat IL-2 Rabbit Recombinant Antibody

IL-2 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Rat

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240264C7

Cat no : 98046-1-RR

Print datasheet

Synonyms

Il2, interleukin 2, 240264C7

验证数据展示

  • FC experiment of rat splenocytes using 98046-1-RR

    FC experiment of rat splenocytes using 98046-1-RR

    1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with APC Anti-Rat CD4 (OX-35) and 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of rat splenocytes using 98046-1-RR

    FC experiment of rat splenocytes using 98046-1-RR

    1x10^6 PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98046-1-RR

    Affinity and Kinetic Characterization of 98046-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98046-1-RR against Rat IL2 were performed. The affinity constant is 0.36 nM.

经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated rat splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98046-1-RR targets IL-2 in FC (Intra) applications and shows reactivity with Rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2
Synonyms Il2, interleukin 2, 240264C7
Calculated Molecular Weight18 kDa
GenBank Accession NumberNM_053836
Gene Symbol Il2
Gene ID (NCBI) 116562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17108
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

实验方案

Product Specific Protocols
FC protocol for IL-2 antibody 98046-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols