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IL-27 Monoclonal antibody, PBS Only

IL-27 Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

1E1H5

Cat No : 66164-1-PBS

Print datasheet

Synonyms

IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha

验证数据展示

  • WB analysis of Recombinant protein using 66164-1-Ig (same clone as 66164-1-PBS)

    WB analysis of Recombinant protein using 66164-1-Ig (same clone as 66164-1-PBS)

    IL27 fusion protein were subjected to SDS PAGE followed by western blot with 66164-1-Ig (IL-27 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66164-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66164-1-Ig (same clone as 66164-1-PBS)

    IHC staining of human placenta using 66164-1-Ig (same clone as 66164-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66164-1-Ig (IL-27 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66164-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 66164-1-Ig (same clone as 66164-1-PBS)

    IHC staining of human placenta using 66164-1-Ig (same clone as 66164-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66164-1-Ig (IL-27 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66164-1-PBS in a different storage buffer formulation.

产品信息

66164-1-PBS targets IL-27 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity human
Immunogen IL-27 fusion protein Ag21436 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name interleukin 27
Synonyms IL 27, IL 27 A, IL 27 subunit alpha, IL 27A, IL27, IL-27, IL27 A, IL27A, IL27p28, IL30, interleukin 27, Interleukin 27 subunit alpha
Calculated Molecular Weight 243 aa, 27 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC062422
Gene Symbol IL27
Gene ID (NCBI) 246778
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NEV9
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.