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IL-3 Recombinant antibody, PBS Only (Capture/Detector)

IL-3 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240417G4

Cat no : 83544-4-PBS

Print datasheet

Synonyms

IL3, Multipotential colony-stimulating factor, Interleukin-3, Interleukin 3, IL 3

验证数据展示

  • Sandwich ELISA standard curve of MP00526-4

    Sandwich ELISA standard curve of MP00526-4

    Sandwich ELISA standard curve of MP00526-1, IL-3 Recombinant Matched Antibody Pair - PBS only. 83544-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0829.83544-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00526-4

    Sample test of MP00526-4

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean IL-3 concentration was determined to be 43.17 pg/mL in unstimulated PBMC supernatant, 676.99 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP00526-4

    Sample test of MP00526-4

    Serum of seven individual healthy human donors was measured. The IL-3 concentration of detected samples was determined to be 20.87 pg/mL with a range of 5.19 - 65.92 pg/mL.

  • Cytometric bead array standard curve of MP00526-1

    Cytometric bead array standard curve of MP00526-1

    Cytometric bead array standard curve of MP00526-1, IL-3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83544-1-PBS. Detection antibody: 83544-4-PBS. Standard: Eg0829. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00526-3

    Cytometric bead array standard curve of MP00526-3

    Cytometric bead array standard curve of MP00526-3, IL-3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83544-4-PBS. Detection antibody: 83544-2-PBS. Standard: Eg0829. Range: 0.313-20 ng/mL.

产品信息

83544-4-PBS targets IL-3 as part of a matched antibody pair:

MP00526-1: 83544-1-PBS capture and 83544-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00526-3: 83544-4-PBS capture and 83544-2-PBS detection (validated in Cytometric bead array)

MP00526-4: 83544-4-PBS capture and 83544-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 3 (colony-stimulating factor, multiple)
Synonyms IL3, Multipotential colony-stimulating factor, Interleukin-3, Interleukin 3, IL 3
Calculated Molecular Weight17 kDa
GenBank Accession NumberNM_000588.3
Gene Symbol IL-3
Gene ID (NCBI) 3562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08700
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C