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IL-4 Recombinant antibody

IL-4 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240794A8

Cat no : 83894-1-RR

Print datasheet

Synonyms

IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1

验证数据展示

  • IF Staining of Ramos using 83894-1-RR

    IF Staining of Ramos using 83894-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using IL-4 antibody (83894-1-RR, Clone: 240794A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83894-1-RR

    Affinity and Kinetic Characterization of 83894-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83894-1-RR against Human IL-4 were performed. The affinity constant is 1.41 nM.

经过测试的应用

Positive IF/ICC detected inRamos cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83894-1-RR is IL-4 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 4
Synonyms IL4, interleukin 4, IL 4, BSF-1, B-cell stimulatory factor 1
Calculated Molecular Weight 153 aa, 17 kDa
GenBank Accession NumberBC070123
Gene Symbol IL-4
Gene ID (NCBI) 3565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

实验方案

Product Specific Protocols
IF protocol for IL-4 antibody 83894-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols