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IL-6 Recombinant antibody

IL-6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240670F10

Cat no : 83747-5-RR

Print datasheet

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

验证数据展示

  • WB analysis of HUVEC using 83747-5-RR

    WB analysis of HUVEC using 83747-5-RR

    LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83747-5-RR (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 83747-5-RR

    IF Staining of THP-1 using 83747-5-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL-6 antibody (83747-5-RR, Clone: 240670F10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83747-5-RR

    Affinity and Kinetic Characterization of 83747-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83747-5-RR against Human IL-6 were performed. The affinity constant is 1.60 nM.

经过测试的应用

Positive WB detected inLPS and Brefeldin A treated HUVEC cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83747-5-RR is IL-6 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 (interferon, beta 2)
Synonyms IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05231
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

实验方案

Product Specific Protocols
WB protocol for IL-6 antibody 83747-5-RRDownload protocol
IF protocol for IL-6 antibody 83747-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols