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IL-6R alpha Monoclonal antibody, PBS Only

IL-6R alpha Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

2A5F4

Cat No : 66855-1-PBS

Print datasheet

Synonyms

CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL-6R, IL-6R alpha, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80

验证数据展示

  • WB analysis of human spleen using 66855-1-Ig (same clone as 66855-1-PBS)

    WB analysis of human spleen using 66855-1-Ig (same clone as 66855-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

产品信息

66855-1-PBS targets IL-6R alpha in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity Human
Immunogen IL-6R alpha fusion protein Ag18263 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interleukin 6 receptor
Synonyms CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL-6R, IL-6R alpha, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80
Calculated Molecular Weight 468 aa, 52 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC132684
Gene Symbol IL6R
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08887
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).