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Host
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis of human spleen using 66855-1-Ig (same clone as 66855-1-PBS)

    WB analysis of human spleen using 66855-1-Ig (same clone as 66855-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

CD126/IL-6R alpha Monoclonal antibody, PBS Only

CD126/IL-6R alpha Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66855-1-PBS

产品说明书

CloneNo. 2A5F4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

CD126, IL-6R alpha, 2A5F4, IL 6R alpha, IL 6R 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66855-1-PBS targets CD126/IL-6R alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD126/IL-6R alpha fusion protein Ag18263 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 6 receptor
别名 CD126, IL-6R alpha, 2A5F4, IL 6R alpha, IL 6R 1
计算分子量 468 aa, 52 kDa
观测分子量 80 kDa
GenBank蛋白编号BC132684
基因名称 IL-6R alpha
Gene ID (NCBI) 3570
RRIDAB_2882195
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08887
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).