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验证数据展示

  • WB analysis of Recombinant protein using 66144-1-Ig (same clone as 66144-1-PBS)

    WB analysis of Recombinant protein using 66144-1-Ig (same clone as 66144-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 66144-1-Ig (IL9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    IF Staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-9 antibody (66144-1-Ig, Clone: 1F3A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

IL-9 Monoclonal antibody, PBS Only

IL-9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66144-1-PBS

产品说明书

CloneNo. 1F3A11

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

IL9, Interleukin-9, interleukin9, interleukin 9, IL 9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66144-1-PBS targets IL-9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 IL-9 fusion protein Ag21435 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 9
别名 IL9, Interleukin-9, interleukin9, interleukin 9, IL 9
计算分子量 144 aa, 16 kDa
观测分子量 19 kDa
GenBank蛋白编号BC066284
基因名称 IL9
Gene ID (NCBI) 3578
RRIDAB_2881541
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15248
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.