验证数据展示
经过测试的应用
| Positive WB detected in | rat skeletal muscle tissue, Jurkat cells, mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
15102-1-AP targets IL-10RB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | IL-10RB fusion protein Ag7445 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 10 receptor, beta |
| 别名 | CDW210B, CRF2 4, CRFB4, D21S58, D21S66, IL 10 receptor subunit beta, IL 10R subunit 2, IL 10R subunit beta, IL 10R2, IL 10RB, IL10RB, IL-10RB, IL-10Rβ, interleukin 10 receptor, beta |
| 计算分子量 | 37 kDa |
| 观测分子量 | 42-45 kDa |
| GenBank蛋白编号 | BC001903 |
| 基因名称 | IL10RB |
| Gene ID (NCBI) | 3588 |
| RRID | AB_10638926 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08334 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RB, also known as IL-10R2 or CDw210b, is the signaling subunit of the IL-10R complex and is constitutively expressed in a variety of cell types (PMID: 24507158). Binding of IL-10 to the IL-10R complex leads to JAK1- and TYK2-mediated phosphorylation of STAT3 (PMID: 11244051; 10433356). IL-10RB is also shared by several other cytokines, including IL22, IL-26, IFN-γ, IL-28A/B and IL29 (PMID: 22236434).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-10RB antibody 15102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Macrophage-Derived Nanosponges Adsorb Cytokines and Modulate Macrophage Polarization for Renal Cell Carcinoma Immunotherapy | ||
Biochim Biophys Acta Mol Basis Dis IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C. | ||
PLoS One Endogenous interleukin-22 prevents cardiac rupture after myocardial infarction in mice |
