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IL-11RA Polyclonal antibody

IL-11RA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10264-1-AP

Print datasheet

Synonyms

IL11RA, IL-11R subunit alpha, IL-11 receptor subunit alpha, IL 11RA, IL 11R subunit alpha

验证数据展示

  • WB analysis using 10264-1-AP

    WB analysis using 10264-1-AP

    Mouse colon tissue was subjected to SDS PAGE followed by western blot with 10264-1-AP (IL-11RA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of N2a cells using 10264-1-AP

    IF Staining of N2a cells using 10264-1-AP

    IF result of anti-IL11RA (10264-1-AP, 1:200) with N2a cells (4%PFA fixed) by Miss Masue Marbiah, PhD student MRC Prion Unit, Department of Neurodegenerative Diseases, UCL Institute of Neurology.

  • IHC staining of human prostate cancer using 10264-1-AP

    IHC staining of human prostate cancer using 10264-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 10264-1-AP

    IHC staining of human prostate cancer using 10264-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10264-1-AP (IL-11RA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman prostate cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inN2a cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10264-1-AP targets IL-11RA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen IL-11RA fusion protein Ag0373 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 11 receptor, alpha
Synonyms IL11RA, IL-11R subunit alpha, IL-11 receptor subunit alpha, IL 11RA, IL 11R subunit alpha
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC003110
Gene Symbol IL11RA
Gene ID (NCBI) 3590
RRIDAB_2125660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-11 (IL-11), a member of the IL-6 family of cytokines, was firstly identified in bone marrow-derived stromal cells (PMID: 2145578). IL-11 is a multifunctional cytokine that plays important roles in hematopoiesis, bone development, tissue repair, and tumor development (PMID: 33863879). IL-10 signals through a receptor complex consisting of IL-11 receptor alpha subunit (IL-11RA) and gp130. IL-11RA binds IL-11 and gp130 transmits signals to the nucleus via Janus kinase (JAK) activation. In addition to a membrane-bound form, IL-11RA can also exist as a soluble form (sIL-11RA) that acts as an agonist of IL-11 activity (PMID: 26876177; 27493449).

实验方案

Product Specific Protocols
WB protocol for IL-11RA antibody 10264-1-APDownload protocol
IHC protocol for IL-11RA antibody 10264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

EMBO J

Identification of a gene regulatory network associated with prion replication.

Authors - Masue M Marbiah
View Article
mouseWB

ACS Nano

Nanoparticle-Mediated RNA Therapy Attenuates Nonalcoholic Steatohepatitis and Related Fibrosis by Targeting Activated Hepatic Stellate Cells

Authors - Chenshuang Zhang
View Article