验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue |
| Positive IHC detected in | human prostate cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | N2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
10264-1-AP targets IL-11RA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | IL-11RA fusion protein Ag0373 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 11 receptor, alpha |
| 别名 | IL11RA, IL-11R subunit alpha, IL-11 receptor subunit alpha, IL 11RA, IL 11R subunit alpha |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC003110 |
| 基因名称 | IL11RA |
| Gene ID (NCBI) | 3590 |
| RRID | AB_2125660 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14626 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-11 (IL-11), a member of the IL-6 family of cytokines, was firstly identified in bone marrow-derived stromal cells (PMID: 2145578). IL-11 is a multifunctional cytokine that plays important roles in hematopoiesis, bone development, tissue repair, and tumor development (PMID: 33863879). IL-10 signals through a receptor complex consisting of IL-11 receptor alpha subunit (IL-11RA) and gp130. IL-11RA binds IL-11 and gp130 transmits signals to the nucleus via Janus kinase (JAK) activation. In addition to a membrane-bound form, IL-11RA can also exist as a soluble form (sIL-11RA) that acts as an agonist of IL-11 activity (PMID: 26876177; 27493449).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-11RA antibody 10264-1-AP | Download protocol |
| IHC protocol for IL-11RA antibody 10264-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Nanoparticle-Mediated RNA Therapy Attenuates Nonalcoholic Steatohepatitis and Related Fibrosis by Targeting Activated Hepatic Stellate Cells |
