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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 30756-1-AP

    WB analysis using 30756-1-AP

    Recombinant protein Eg0742 (IL-12 p70) was subjected to SDS PAGE followed by western blot with 30756-1-AP (IL12(IL12B+IL12A) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL-12 p70 Polyclonal antibody

IL-12 p70 Polyclonal Antibody for WB, ELISA
Cat No. 30756-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, ELISA

别名

IL12, IL-12, IL-12 p70

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30756-1-AP targets IL-12 p70 in WB, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IL-12 p70
别名 IL12, IL-12, IL-12 p70
计算分子量70 kDa
观测分子量 70 kDa
基因名称
Gene ID (NCBI)
RRIDAB_3086410
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43432(IL12B);P43431(IL12A)
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.

实验方案

Product Specific Protocols
WB protocol for IL-12 p70 antibody 30756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols