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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 30756-1-AP

    WB analysis using 30756-1-AP

    Recombinant protein Eg0742 (IL-12 p70) was subjected to SDS PAGE followed by western blot with 30756-1-AP (IL12(IL12B+IL12A) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL-12 p70 Polyclonal antibody

IL-12 p70 Polyclonal Antibody for WB, ELISA
Cat No. 30756-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

IL12, IL-12, IL-12 p70

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30756-1-AP targets IL-12 p70 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IL-12 p70
Synonyms IL12, IL-12, IL-12 p70
Calculated Molecular Weight70 kDa
Observed Molecular Weight 70 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_3086410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43432(IL12B);P43431(IL12A)
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.

实验方案

Product Specific Protocols
WB protocol for IL-12 p70 antibody 30756-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols