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验证数据展示

  • Sandwich ELISA standard curve of MP01262-2

    Sandwich ELISA standard curve of MP01262-2

    Sandwich ELISA standard curve of MP01262-2, Human IL12RB1 Recombinant Matched Antibody Pair - PBS only. 84391-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00005. 84391-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP01262-2

    Sample test of MP01262-2

    The mean IL12RB1 concentration was determined to be 27.14 ng/mL in Jurkat cell extract based on a 1.9 mg/mL extract load and 48.02 ng/mL in THP-1 cell extract based on a 1.5 mg/mL extract load.

  • IF Staining of K-562 using 84391-4-RR (same clone as 84391-4-PBS)

    IF Staining of K-562 using 84391-4-RR (same clone as 84391-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84391-4-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 84391-4-RR (same clone as 84391-4-PBS)

    IF Staining of THP-1 using 84391-4-RR (same clone as 84391-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL12RB1 antibody (84391-4-RR, Clone: 241661D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84391-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01262-2

    Cytometric bead array standard curve of MP01262-2

    Cytometric bead array standard curve of MP01262-2, IL12RB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84391-3-PBS. Detection antibody: 84391-4-PBS. Standard: RP00005. Range: 0.781-50 ng/mL.

IL12RB1 Recombinant antibody, PBS Only (Detector)

IL12RB1 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84391-4-PBS

产品说明书

CloneNo. 241661D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1, CD212

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84391-4-PBS targets IL12RB1 as part of a matched antibody pair:

MP01262-2: 84391-3-PBS capture and 84391-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 12 receptor, beta 1
别名 IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1, CD212
计算分子量 662 aa, 73 kDa
GenBank蛋白编号BC029121
基因名称 IL12RB1
Gene ID (NCBI) 3594
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42701
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.