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验证数据展示

  • Sandwich ELISA standard curve of MP01289-3

    Sandwich ELISA standard curve of MP01289-3

    Sandwich ELISA standard curve of MP01289-3, Human IL-1 alpha Recombinant Matched Antibody Pair - PBS only. 84411-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1320. 84411-3-PBS was HRP conjugated as the detection antibody. Range: 9.77-313 pg/mL.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean IL-1 alpha concentration was determined to be 2.1 pg/mL in unstimulated PBMC supernatant, 880.0 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    The mean IL-1 alpha concentration was determined to be 271.0 ng/mL in mouse liver cell extract based on a 3.7 mg/mL extract load.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    Serum of five individual healthy human donors was measured. The IL-1 alpha concentration of detected samples was determined to be 5.0 pg/mL with a range of 1.8-8.2 pg/mL.

  • Cytometric bead array standard curve of MP01289-1

    Cytometric bead array standard curve of MP01289-1

    Cytometric bead array standard curve of MP01289-1, IL-1 alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84411-2-PBS. Detection antibody: 84411-1-PBS. Standard: HZ-1320. Range: 0.039-10 ng/mL.

IL1A Recombinant antibody, PBS Only (Capture)

IL1A Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84411-2-PBS

产品说明书

CloneNo. 241752E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

IL 1 alpha, IL-1 alpha, IL1 ALPHA, IL 1A, Hematopoietin-1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IL1A Ready-To-Use IHC Kit. IL1A primary antibody included.

产品信息

84411-2-PBS targets IL1A as part of a matched antibody pair:

MP01289-1: 84411-2-PBS capture and 84411-1-PBS detection (validated in Cytometric bead array)

MP01289-3: 84411-2-PBS capture and 84411-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 IL1A fusion protein HZ-1320 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 1, alpha
别名 IL 1 alpha, IL-1 alpha, IL1 ALPHA, IL 1A, Hematopoietin-1
计算分子量 271 aa, 31 kDa
GenBank蛋白编号BC013142
基因名称 IL-1 alpha
Gene ID (NCBI) 3552
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01583
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C