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IL1A Recombinant antibody, PBS Only (Capture/Detector)

IL1A Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241752F11

Cat no : 84411-3-PBS

Print datasheet

Synonyms

IL 1 alpha, IL-1 alpha, IL1 ALPHA, IL 1A, Hematopoietin-1

验证数据展示

  • Sandwich ELISA standard curve of MP01289-3

    Sandwich ELISA standard curve of MP01289-3

    Sandwich ELISA standard curve of MP01289-3, Human IL-1 alpha Recombinant Matched Antibody Pair - PBS only. 84411-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1320. 84411-3-PBS was HRP conjugated as the detection antibody. Range: 9.77-313 pg/mL.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean IL-1 alpha concentration was determined to be 2.1 pg/mL in unstimulated PBMC supernatant, 880.0 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    The mean IL-1 alpha concentration was determined to be 271.0 ng/mL in mouse liver cell extract based on a 3.7 mg/mL extract load.

  • Sample test of MP01289-3

    Sample test of MP01289-3

    Serum of five individual healthy human donors was measured. The IL-1 alpha concentration of detected samples was determined to be 5.0 pg/mL with a range of 1.8-8.2 pg/mL.

  • Cytometric bead array standard curve of MP01289-2

    Cytometric bead array standard curve of MP01289-2

    Cytometric bead array standard curve of MP01289-2, IL-1 alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84411-3-PBS. Detection antibody: 84411-4-PBS. Standard: HZ-1320. Range: 0.078-10 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy IL1A Ready-To-Use IHC Kit. IL1A primary antibody included.

产品信息

84411-3-PBS targets IL1A as part of a matched antibody pair:

MP01289-2: 84411-3-PBS capture and 84411-4-PBS detection (validated in Cytometric bead array)

MP01289-3: 84411-2-PBS capture and 84411-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen IL1A fusion protein HZ-1320 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 1, alpha
Synonyms IL 1 alpha, IL-1 alpha, IL1 ALPHA, IL 1A, Hematopoietin-1
Calculated Molecular Weight 271 aa, 31 kDa
GenBank Accession NumberBC013142
Gene Symbol IL-1 alpha
Gene ID (NCBI) 3552
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01583
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C