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验证数据展示

  • WB analysis using 30966-1-AP

    WB analysis using 30966-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30966-1-AP (IL-1RAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30966-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 30966-1-AP

    IHC staining of human placenta using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 30966-1-AP

    IHC staining of human placenta using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 30966-1-AP

    IHC staining of human ovary cancer using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-1RAP Polyclonal antibody

IL-1RAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30966-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

IL1RAP, IL-1 receptor accessory protein, IL 1RAcP, IL 1R3, IL 1R 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, mouse liver tissue
Positive IHC detected inhuman placenta tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30966-1-AP targets IL-1RAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 IL-1RAP fusion protein Ag34121 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 1 receptor accessory protein
别名 IL1RAP, IL-1 receptor accessory protein, IL 1RAcP, IL 1R3, IL 1R 3
计算分子量65 kDa
观测分子量70 kDa
GenBank蛋白编号NM_001167928.1
基因名称 IL1RAP
Gene ID (NCBI) 3556
RRIDAB_3669798
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NPH3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-1RAP (Interleukin-1 receptor accessory protein), also known as IL-1RAcP, IL-1R-3, or IL-1R3, is a member of the interleukin-1 receptor family. IL-1RAP is composed of an extracellular region containing three Ig-like C2 type domains, a transmembrane region, and a cytoplasmic region with a TIR (Toll-IL-1-receptor) domain. IL-1RAP functions as a co-receptor for IL-1, IL-33, IL-36. It plays an essential role in the signaling of the IL-1 family cytokines such as IL-1, IL-33, and IL-36, as well as tyrosine kinases FLT3 and c-Kit. IL-1RAP can also exist in a soluble form.

实验方案

Product Specific Protocols
WB protocol for IL-1RAP antibody 30966-1-APDownload protocol
IHC protocol for IL-1RAP antibody 30966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols