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IL-1RAP Polyclonal antibody

IL-1RAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30966-1-AP

Print datasheet

Synonyms

IL1RAP, IL-1 receptor accessory protein, IL 1RAcP, IL 1R3, IL 1R 3

验证数据展示

  • WB analysis using 30966-1-AP

    WB analysis using 30966-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30966-1-AP (IL-1RAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30966-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 30966-1-AP

    IHC staining of human placenta using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 30966-1-AP

    IHC staining of human placenta using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 30966-1-AP

    IHC staining of human ovary cancer using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, mouse liver tissue
Positive IHC detected inhuman placenta tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30966-1-AP targets IL-1RAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen IL-1RAP fusion protein Ag34121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 1 receptor accessory protein
Synonyms IL1RAP, IL-1 receptor accessory protein, IL 1RAcP, IL 1R3, IL 1R 3
Calculated Molecular Weight65 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_001167928.1
Gene Symbol IL1RAP
Gene ID (NCBI) 3556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9NPH3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-1RAP (Interleukin-1 receptor accessory protein), also known as IL-1RAcP, IL-1R-3, or IL-1R3, is a member of the interleukin-1 receptor family. IL-1RAP is composed of an extracellular region containing three Ig-like C2 type domains, a transmembrane region, and a cytoplasmic region with a TIR (Toll-IL-1-receptor) domain. IL-1RAP functions as a co-receptor for IL-1, IL-33, IL-36. It plays an essential role in the signaling of the IL-1 family cytokines such as IL-1, IL-33, and IL-36, as well as tyrosine kinases FLT3 and c-Kit. IL-1RAP can also exist in a soluble form.

实验方案

Product Specific Protocols
WB protocol for IL-1RAP antibody 30966-1-APDownload protocol
IHC protocol for IL-1RAP antibody 30966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols