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验证数据展示

  • WB analysis of MDA-MB-453s using 22861-1-AP

    WB analysis of MDA-MB-453s using 22861-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22861-1-AP (IL22RA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 22861-1-AP

    IHC staining of human lung cancer using 22861-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 22861-1-AP

    IHC staining of human lung cancer using 22861-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-22RA2 Polyclonal antibody

IL-22RA2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22861-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22861-1-AP targets IL-22RA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 IL-22RA2 fusion protein Ag18854 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 22 receptor, alpha 2
别名 CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16
计算分子量 263 aa, 31 kDa
观测分子量 27 kDa
GenBank蛋白编号BC125167
基因名称 IL-22RA2
Gene ID (NCBI) 116379
RRIDAB_2879180
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969J5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-22 receptor subunit alpha-2 (IL22RA2, also known as CRF2-10) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. It has been described that human IL22RA2 gene encodes three alternatively spliced isoforms called CRF2-10L, CRF2-10, and CRF2-10s, with molecular weights of 31 kDa, 27 kDa, and 15 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for IL-22RA2 antibody 22861-1-APDownload protocol
IHC protocol for IL-22RA2 antibody 22861-1-APDownload protocol
FC protocol for IL-22RA2 antibody 22861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols