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IL-23R Polyclonal antibody

IL-23R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27163-1-AP

Print datasheet

Synonyms

IL 23 receptor, IL 23R, IL23R, IL-23R, interleukin 23 receptor

验证数据展示

  • WB analysis using 27163-1-AP

    WB analysis using 27163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27163-1-AP (IL23R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27163-1-AP

    IHC staining of human tonsillitis using 27163-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 27163-1-AP

    IHC staining of human tonsillitis using 27163-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27163-1-AP (IL23R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, A431 cells, RAW 264.7 cells, Raji cells, THP-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 27163-1-AP is IL-23R Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Immunogen IL-23R fusion protein Ag25939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 23 receptor
Synonyms IL 23 receptor, IL 23R, IL23R, IL-23R, interleukin 23 receptor
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession Number NM_144701
Gene Symbol IL-23R
Gene ID (NCBI) 149233
RRIDAB_2880783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IL-23R antibody 27163-1-APDownload protocol
IHC protocol for IL-23R antibody 27163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

IL-17 Aggravates Pseudomonas aeruginosa Airway Infection in Acute Exacerbations of Chronic Obstructive Pulmonary Disease.

Authors - Fengming Ding
View Article
ratWB

Naunyn Schmiedebergs Arch Pharmacol

Short-term pretreatment of naringin isolated from Citrus wilsonii Tanaka attenuates rat myocardial ischemia/reperfusion injury.

Authors - Wenting Liu
View Article