验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, K-562 cells, TF-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12308-1-AP targets IL-27RA in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | IL-27RA fusion protein Ag2956 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 27 receptor, alpha |
| 别名 | IL27RA, IL 27R alpha, IL 27 receptor subunit alpha, Cytokine receptor-like 1, Cytokine receptor WSX-1 |
| 计算分子量 | 636 aa, 69 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC028003 |
| 基因名称 | IL27RA |
| Gene ID (NCBI) | 9466 |
| RRID | AB_3669136 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UWB1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-27RA, also known as T-cell cytokine receptor WSX-1 or TCCR, is a unique receptor subunit for the cytokine IL-27. It is expressed in a variety of cells including activated T cells, NK cells, mast cells, endothelial cells, activated B cells, monocytes, Langerhans cells, and activated dendritic cells (PMID: 18378322). The interaction with IL-27 leads to the phosphorylation of Jak and STAT proteins, which are crucial for the regulation of immune responses (PMID: 28536468). IL-27RA plays a significant role in immune cell activation and differentiation, particularly in the context of Th1 and Th17 cells (PMID: 38566992).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-27RA antibody 12308-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |