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验证数据展示

  • WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

    WB analysis of MJ using 83896-1-RR (same clone as 83896-1-PBS)

    MJ cells were subjected to SDS PAGE followed by western blot with 83896-1-RR (IL2RA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83896-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00837-2

    Cytometric bead array standard curve of MP00837-2

    Cytometric bead array standard curve of MP00837-2, CD25/IL-2RA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83896-2-PBS. Detection antibody: 83896-1-PBS. Standard: Eg0408. Range: 2.5-40 ng/mL.

  • Affinity and Kinetic Characterization of 83896-1-RR

    Affinity and Kinetic Characterization of 83896-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83896-1-RR against Human CD25/IL2RA were performed. The affinity constant is 31.7 nM.

CD25/IL-2RA Recombinant antibody, PBS Only (Detector)

CD25/IL-2RA Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 83896-1-PBS

产品说明书

CloneNo. 240906A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

CD25, 240906A12, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83896-1-PBS targets CD25/IL-2RA as part of a matched antibody pair:

MP00837-2: 83896-2-PBS capture and 83896-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 2 receptor, alpha
别名 CD25, 240906A12, IL2 RA, IL 2R subunit alpha, IL 2 receptor subunit alpha
计算分子量 272 aa, 31 kDa
观测分子量60 kDa
GenBank蛋白编号BC156627
基因名称 IL2RA
Gene ID (NCBI) 3559
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01589
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).