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IL3RA/CD123 Recombinant antibody, PBS Only (Capture)

IL3RA/CD123 Recombinant Antibody for Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240042F5

Cat No : 83243-2-PBS

Print datasheet

Synonyms

CD123, hIL 3Ra, IL 3 receptor subunit alpha, IL 3R alpha, IL 3R subunit alpha, IL 3RA, IL3R, IL3RA, IL3RAY, IL3RX, IL3RY

验证数据展示

  • Cytometric bead array standard curve of MP00176-1

    Cytometric bead array standard curve of MP00176-1

    Cytometric bead array standard curve ofMP00176-1, IL3RA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83243-2-PBS. Detection antibody: 83243-1-PBS. Standard: Ag4452. Range: 1.25-20 ng/mL.

产品信息

83243-2-PBS targets IL3RA/CD123 as part of a matched antibody pair:

MP00176-1: 83243-2-PBS capture and 83243-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen IL3RA/CD123 fusion protein Ag4452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 3 receptor, alpha (low affinity)
Synonyms CD123, hIL 3Ra, IL 3 receptor subunit alpha, IL 3R alpha, IL 3R subunit alpha, IL 3RA, IL3R, IL3RA, IL3RAY, IL3RX, IL3RY
Calculated Molecular Weight 378 aa, 43 kDa
GenBank Accession NumberBC035407
Gene Symbol IL3RA
Gene ID (NCBI) 3563
ENSEMBL Gene IDENSG00000185291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26951
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Interleukin-3 receptor subunit alpha (IL3RA), also known as CD123, belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). IL3RA is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to IL3RA and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.