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IL-5 Polyclonal antibody

IL-5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26677-1-AP

Print datasheet

Synonyms

EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF

验证数据展示

  • IHC staining of human breast cancer using 26677-1-AP

    IHC staining of human breast cancer using 26677-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26677-1-AP

    IHC staining of human breast cancer using 26677-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26677-1-AP (IL5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Recombinant protein using 26677-1-AP

    WB analysis of Recombinant protein using 26677-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 26677-1-AP (IL5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26677-1-AP is IL-5 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen IL-5 fusion protein Ag24583 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 5 (colony-stimulating factor, eosinophil)
Synonyms EDF, IL 5, IL5, IL-5, Interleukin 5, T cell replacing factor, TRF
Calculated Molecular Weight 134 aa, 15 kDa
GenBank Accession NumberBC066282
Gene Symbol IL5
Gene ID (NCBI) 3567
RRIDAB_2880598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05113
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IL-5 antibody 26677-1-APDownload protocol
IHC protocol for IL-5 antibody 26677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Laryngoscope

Eosinophilic Sialodochitis: A Type of Chronic Obstructive Sialadenitis Related to Allergy.

Authors - Wen-Xuan Zhu
View Article
humanIHC

Laryngoscope

Primary Hyper-IgE-Related Salivary Gland Disease: A New Disease Entity.

Authors - Wen-Xuan Zhu
View Article
humanWB

J Immunol Res

Respiratory Syncytial Virus Nonstructural Protein 1 Promotes 5-Lipoxygenase via miR-19a-3p.

Authors - Mei Zheng
View Article