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CD126/IL-6R alpha Polyclonal antibody

CD126/IL-6R alpha Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 23457-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (1)

CD126, IL-6R alpha, IL 6R subunit alpha, IL 6R alpha, IL 6R 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse spleen tissue, Jurkat cells, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23457-1-AP targets CD126/IL-6R alpha in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 CD126/IL-6R alpha fusion protein Ag18263 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 6 receptor
别名 CD126, IL-6R alpha, IL 6R subunit alpha, IL 6R alpha, IL 6R 1
计算分子量 468 aa, 52 kDa
观测分子量 80 kDa
GenBank蛋白编号BC132684
基因名称 IL-6R alpha
Gene ID (NCBI) 3570
RRIDAB_2827428
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08887
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

实验方案

Product Specific Protocols
WB protocol for CD126/IL-6R alpha antibody 23457-1-APDownload protocol
IHC protocol for CD126/IL-6R alpha antibody 23457-1-APDownload protocol
IP protocol for CD126/IL-6R alpha antibody 23457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Signal Transduct Target Ther

Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms

Authors - Na Jia
mouseWB

Cancer Discov

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Authors - Wan Du
humanIF

J Med Virol

Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis

Authors - Arun Das
human,mouseIF

Acta Pharmacol Sin

Exosomes derived from induced cardiopulmonary progenitor cells alleviate acute lung injury in mice

Authors - Luo-Xing Xia
mouseWB

Allergol Int

Prevention of IL-6 signaling ameliorates toluene diisocyanate-induced steroid-resistant asthma.

Authors - Shuyu Chen
humanWB

Pharmacol Res

Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin.

Authors - Long Wang
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