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验证数据展示

  • Sandwich ELISA standard curve of MP00676-1

    Sandwich ELISA standard curve of MP00676-1

    Sandwich ELISA standard curve of MP00676-1, Human IL6R/IL-6R alpha Recombinant Matched Antibody Pair - PBS only. 83688-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0490. 83688-2-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

  • Sample test of MP00676-1

    Sample test of MP00676-1

    Urine of eight humans was measured. The human IL6R/IL-6R alpha concentration of these samples was determined to be 1,291.5 pg/mL, with a range of 119.4 - 5,099.9 pg/mL.

  • Sample test of MP00676-1

    Sample test of MP00676-1

    Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1day, 4 days and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL6.

  • Sample test of MP00676-1

    Sample test of MP00676-1

    Serum of sixteen humans was measured. The human IL6R/IL-6R alpha concentration of these samples was determined to be 60,089 pg/mL, with a range of 37,850.6 - 124,335.2 pg/mL.

  • Cytometric bead array standard curve of MP00676-1

    Cytometric bead array standard curve of MP00676-1

    Cytometric bead array standard curve of MP00676-1, CD126/IL-6R alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83688-3-PBS. Detection antibody: 83688-2-PBS. Standard: Eg0490. Range: 0.625-80 ng/mL.

CD126/IL-6R alpha Recombinant antibody, PBS Only (Capture)

CD126/IL-6R alpha Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83688-3-PBS

产品说明书

CloneNo. 240734A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

CD126, IL-6R alpha, 240734A8, IL 6R alpha, IL 6R 1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83688-3-PBS targets CD126/IL-6R alpha as part of a matched antibody pair:

MP00676-1: 83688-3-PBS capture and 83688-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 6 receptor
别名 CD126, IL-6R alpha, 240734A8, IL 6R alpha, IL 6R 1
计算分子量 468 aa, 52 kDa
GenBank蛋白编号BC132684
基因名称 IL-6R alpha
Gene ID (NCBI) 3570
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08887
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C