gp130/IL6ST Recombinant antibody, PBS Only (Detector)

Sandwich ELISA standard curve of MP00324-3, Human gp130 Recombinant Matched Antibody Pair - PBS only. 83284-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0024. 83284-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

Serum of sixteen individual healthy human donors was measured. The human gp130 concentration of detected samples was determined to be 123.3 ng/mL with a range of 38.7 - 330.5 ng/mL.

Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of human gp130.