• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ILK Polyclonal antibody, PBS Only

ILK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24926-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

    IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

    IHC staining of human pancreas cancer using 24926-1-AP (same clone as 24926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24926-1-AP (ILK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse heart using 24926-1-AP (same clone as 24926-1-PBS)

    WB analysis of mouse heart using 24926-1-AP (same clone as 24926-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 24926-1-AP (ILK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 24926-1-AP (same clone as 24926-1-PBS)

    IF Staining of MCF-7 using 24926-1-AP (same clone as 24926-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 24926-1-AP (ILK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24926-1-PBS in a different storage buffer formulation.

产品信息

24926-1-PBS targets ILK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ILK fusion protein Ag21494 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin-linked kinase
Synonyms
Calculated Molecular Weight 451 aa, 59 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC001554
Gene Symbol ILK
Gene ID (NCBI) 3611
RRIDAB_2879800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13418
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).