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ILVBL Polyclonal antibody

ILVBL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11220-1-AP

Print datasheet

Synonyms

HACL2, EC:4.1.2.-, AHAS, Acetolactate synthase-like protein, 2-hydroxyacyl-CoA lyase 2

验证数据展示

  • WB analysis using 11220-1-AP

    WB analysis using 11220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11220-1-AP

    IF Staining of HeLa using 11220-1-AP

    Immunofluorescent analysis of Hela cells, using ILVBL antibody 11220-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human colon cancer using 11220-1-AP

    IHC staining of human colon cancer using 11220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, human thyroid gland tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11220-1-AP targets ILVBL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ILVBL fusion protein Ag1729 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ilvB (bacterial acetolactate synthase)-like
Synonyms HACL2, EC:4.1.2.-, AHAS, Acetolactate synthase-like protein, 2-hydroxyacyl-CoA lyase 2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC011761
Gene Symbol ILVBL
Gene ID (NCBI) 10994
RRIDAB_2127187
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA1L0T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ILVBL antibody 11220-1-APDownload protocol
IHC protocol for ILVBL antibody 11220-1-APDownload protocol
IF protocol for ILVBL antibody 11220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols