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验证数据展示

  • WB analysis using 11220-1-AP

    WB analysis using 11220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11220-1-AP

    IHC staining of human colon cancer using 11220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11220-1-AP

    IF Staining of HeLa using 11220-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ILVBL antibody (11220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ILVBL Polyclonal antibody

ILVBL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11220-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

HACL2, EC:4.1.2.-, AHAS, Acetolactate synthase-like protein, 2-hydroxyacyl-CoA lyase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, human thyroid gland tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11220-1-AP targets ILVBL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ILVBL fusion protein Ag1729 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ilvB (bacterial acetolactate synthase)-like
别名 HACL2, EC:4.1.2.-, AHAS, Acetolactate synthase-like protein, 2-hydroxyacyl-CoA lyase 2
计算分子量 68 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC011761
基因名称 ILVBL
Gene ID (NCBI) 10994
RRIDAB_2127187
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA1L0T0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ILVBL antibody 11220-1-APDownload protocol
IHC protocol for ILVBL antibody 11220-1-APDownload protocol
IF protocol for ILVBL antibody 11220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols