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IMP5 Polyclonal antibody

IMP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12664-1-AP

Print datasheet

Synonyms

SPPL2c, SPP like 2C, Signal peptide peptidase-like 2C, IMP-5, IMP 5

验证数据展示

  • WB analysis of HEK-293 using 12664-1-AP

    WB analysis of HEK-293 using 12664-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12664-1-AP

    IP experiment of HEK-293 using 12664-1-AP

    IP result of anti-IMP5 (IP:12664-1-AP, 4ug; Detection:12664-1-AP 1:500) with HEK-293 cells lysate 3600ug.

  • IHC staining of human liver cancer using 12664-1-AP

    IHC staining of human liver cancer using 12664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 12664-1-AP

    IHC staining of human liver cancer using 12664-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 12664-1-AP

    IF Staining of HEK-293 using 12664-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12664-1-AP (IMP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, human liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12664-1-AP targets IMP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen IMP5 fusion protein Ag3345 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intramembrane protease 5
Synonyms SPPL2c, SPP like 2C, Signal peptide peptidase-like 2C, IMP-5, IMP 5
Calculated Molecular Weight 684 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC025401
Gene Symbol IMP5
Gene ID (NCBI) 162540
RRIDAB_2296163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IMP5 antibody 12664-1-APDownload protocol
IHC protocol for IMP5 antibody 12664-1-APDownload protocol
IF protocol for IMP5 antibody 12664-1-APDownload protocol
IP protocol for IMP5 antibody 12664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.

Authors - Roberto Simone
View Article