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验证数据展示

  • WB analysis of human kidney using 16593-1-AP

    WB analysis of human kidney using 16593-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16593-1-AP

    IHC staining of human brain using 16593-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16593-1-AP (IMPA1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 16593-1-AP

    IHC staining of human brain using 16593-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16593-1-AP (IMPA1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16593-1-AP

    IF Staining of HepG2 using 16593-1-AP

    Immunofluorescent analysis of HepG2 cells, using IMPA1 antibody 16593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis using 16593-1-AP

    WB analysis using 16593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IMPA1 Polyclonal antibody

IMPA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16593-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IMPA, IMP1, IMP 1, EC:3.1.3.94, EC:3.1.3.25

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat kidney tissue, human kidney tissue, human brain tissue, mouse kidney tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16593-1-AP targets IMPA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IMPA1 fusion protein Ag9632 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol(myo)-1(or 4)-monophosphatase 1
Synonyms IMPA, IMP1, IMP 1, EC:3.1.3.94, EC:3.1.3.25
Calculated Molecular Weight 277 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC008381
Gene Symbol IMPA1
Gene ID (NCBI) 3612
RRIDAB_2127216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29218
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMPA1 is an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol.

实验方案

Product Specific Protocols
WB protocol for IMPA1 antibody 16593-1-APDownload protocol
IHC protocol for IMPA1 antibody 16593-1-APDownload protocol
IF protocol for IMPA1 antibody 16593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols