验证数据展示
经过测试的应用
Positive WB detected in | rat kidney tissue, human kidney tissue, human brain tissue, mouse kidney tissue |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16593-1-AP targets IMPA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | IMPA1 fusion protein Ag9632 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | inositol(myo)-1(or 4)-monophosphatase 1 |
别名 | IMPA, IMP1, IMP 1, EC:3.1.3.94, EC:3.1.3.25 |
计算分子量 | 277 aa, 30 kDa |
观测分子量 | 30 kDa |
GenBank蛋白编号 | BC008381 |
基因名称 | IMPA1 |
Gene ID (NCBI) | 3612 |
RRID | AB_2127216 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P29218 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IMPA1 is an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol.
实验方案
Product Specific Protocols | |
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WB protocol for IMPA1 antibody 16593-1-AP | Download protocol |
IHC protocol for IMPA1 antibody 16593-1-AP | Download protocol |
IF protocol for IMPA1 antibody 16593-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |