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IMPA1 Polyclonal antibody

IMPA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16593-1-AP

Print datasheet

Synonyms

IMPA, IMP1, IMP 1, EC:3.1.3.94, EC:3.1.3.25

验证数据展示

  • WB analysis of human kidney using 16593-1-AP

    WB analysis of human kidney using 16593-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16593-1-AP

    IHC staining of human brain using 16593-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16593-1-AP (IMPA1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 16593-1-AP

    IHC staining of human brain using 16593-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16593-1-AP (IMPA1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16593-1-AP

    IF Staining of HepG2 using 16593-1-AP

    Immunofluorescent analysis of HepG2 cells, using IMPA1 antibody 16593-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis using 16593-1-AP

    WB analysis using 16593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16593-1-AP (IMPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat kidney tissue, human kidney tissue, human brain tissue, mouse kidney tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16593-1-AP targets IMPA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IMPA1 fusion protein Ag9632 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inositol(myo)-1(or 4)-monophosphatase 1
Synonyms IMPA, IMP1, IMP 1, EC:3.1.3.94, EC:3.1.3.25
Calculated Molecular Weight 277 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC008381
Gene Symbol IMPA1
Gene ID (NCBI) 3612
RRIDAB_2127216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29218
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IMPA1 is an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol.

实验方案

Product Specific Protocols
WB protocol for IMPA1 antibody 16593-1-APDownload protocol
IHC protocol for IMPA1 antibody 16593-1-APDownload protocol
IF protocol for IMPA1 antibody 16593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols