验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse spleen tissue, A431 cells, K-562 cells, Raji cells, rat spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22092-1-AP targets IMPDH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | IMPDH1 fusion protein Ag17297 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IMP (inosine monophosphate) dehydrogenase 1 |
| 别名 | IMPDH-I, IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1 |
| 计算分子量 | 563 aa, 60 kDa |
| 观测分子量 | 53-60 kDa |
| GenBank蛋白编号 | BC033622 |
| 基因名称 | IMPDH1 |
| Gene ID (NCBI) | 3614 |
| RRID | AB_2878992 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20839 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa. This antibody may has cross reaction with IMPDH2 due to their high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| IHC protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| IF protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| IP protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. | ||
Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.
| ||
J Oncol The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses
| ||
Cells Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line. | ||
Int J Biochem Cell Biol Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms. | ||
Clin Chem Lab Med Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies. |
