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IMPDH1 Polyclonal antibody
IMPDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse spleen tissue, A431 cells, K-562 cells, Raji cells, rat spleen tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human ovary tumor tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 22092-1-AP is IMPDH1 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | IMPDH1 fusion protein Ag17297 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | IMP (inosine monophosphate) dehydrogenase 1 |
Synonyms | IMP dehydrogenase 1, IMPD, IMPD 1, IMPD1, IMPDH 1, IMPDH I, IMPDH1, LCA11, RP10, sWSS2608 |
Calculated Molecular Weight | 563 aa, 60 kDa |
Observed Molecular Weight | 53-60 kDa |
GenBank Accession Number | BC033622 |
Gene Symbol | IMPDH1 |
Gene ID (NCBI) | 3614 |
RRID | AB_2878992 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20839 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
IHC protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
IF protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
IP protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. | ||
Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.
| ||
J Oncol The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses
| ||
Cells Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line. | ||
Int J Biochem Cell Biol Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms. | ||
Clin Chem Lab Med Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies. |