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IMPDH1 Polyclonal antibody

IMPDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 22092-1-AP

Print datasheet

Synonyms

IMPDH-I, IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1

验证数据展示

WB analysis of HeLa using 22092-1-AP
WB result of IMPDH1 antibody (22092-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH1 transfected HeLa cells.
WB analysis using 22092-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.
Ready-to-use IHC kits available with IMPDH1 antibody included
IHC staining of human ovary tumor using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.
Ready-to-use IHC kits available with IMPDH1 antibody included
IP experiment of HeLa using 22092-1-AP
IP result of anti-IMPDH1 (IP:22092-1-AP, 4ug; Detection:22092-1-AP 1:2000) with HeLa cells lysate 3200ug.
IF Staining of HeLa using 22092-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22092-1-AP (IMPDH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human tonsillitis using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22092-1-AP (IMPDH1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with IMPDH1 antibody included
IHC staining of human tonsillitis using 22092-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22092-1-AP (IMPDH1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with IMPDH1 antibody included

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Cat No. 22092-1-AP

Size

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经过测试的应用

Positive WB detected in	HeLa cells, mouse spleen tissue, A431 cells, K-562 cells, Raji cells, rat spleen tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human tonsillitis tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy IMPDH1 Ready-To-Use IHC Kit. IMPDH1 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:800-1:3200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 13 publications below
IHC	See 4 publications below
IF	See 5 publications below
IP	See 1 publications below

产品信息

22092-1-AP targets IMPDH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Immunogen	IMPDH1 fusion protein Ag17297 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	IMP (inosine monophosphate) dehydrogenase 1
Synonyms	IMPDH-I, IMPDH 1, IMPD1, IMPD 1, IMP dehydrogenase 1
Calculated Molecular Weight	563 aa, 60 kDa
Observed Molecular Weight	53-60 kDa
GenBank Accession Number	BC033622
Gene Symbol	IMPDH1
Gene ID (NCBI)	3614
RRID	AB_2878992
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P20839
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa. This antibody may has cross reaction with IMPDH2 due to their high homology.

实验方案

Product Specific Protocols
WB protocol for IMPDH1 antibody 22092-1-AP	Download protocol
IHC protocol for IMPDH1 antibody 22092-1-AP	Download protocol
IF protocol for IMPDH1 antibody 22092-1-AP	Download protocol
IP protocol for IMPDH1 antibody 22092-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. Authors - Eunus S Ali View Article
human	WB,IHC,IF,IP	Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors. Authors - Hailong Ruan KD Validated View Article
human	WB	J Oncol The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses Authors - Chang-Jun Liu KD Validated View Article
human	WB	Cells Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line. Authors - Madhu Kollareddy View Article
human	WB	Int J Biochem Cell Biol Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms. Authors - Gunter Jennifer H JH View Article
human	WB	Clin Chem Lab Med Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies. Authors - S John Calise View Article

IMPDH1 Polyclonal antibody

IMPDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

验证数据展示

WB analysis of HeLa using 22092-1-AP

WB analysis using 22092-1-AP

IHC staining of human ovary tumor using 22092-1-AP

IHC staining of human ovary tumor using 22092-1-AP

IP experiment of HeLa using 22092-1-AP

IF Staining of HeLa using 22092-1-AP

IHC staining of human tonsillitis using 22092-1-AP

IHC staining of human tonsillitis using 22092-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses

Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.

Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms.

Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies.