验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rat heart tissue, A549 cells, MCF-7 cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12948-1-AP targets IMPDH2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 | IMPDH2 fusion protein Ag3632 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IMP (inosine monophosphate) dehydrogenase 2 |
| 别名 | IMPDH II, IMPDH 2, IMPD2, IMPD 2, IMP dehydrogenase II |
| 计算分子量 | 514 aa, 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC012840 |
| 基因名称 | IMPDH2 |
| Gene ID (NCBI) | 3615 |
| RRID | AB_2127351 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12268 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) IMPDH2 is highly enriched in RR(filamentous rods and rings). This antibody may have cross-reaction with IMPDH1 due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| IHC protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| IF protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| IP protocol for IMPDH2 antibody 12948-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. | ||
Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.
| ||
Cancer Lett Circular RNA circPFKP promotes cell proliferation by activating IMPDH2 in prostate cancer. | ||
Elife SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome. |
