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验证数据展示

  • WB analysis of human heart using 16185-1-AP

    WB analysis of human heart using 16185-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16185-1-AP (ING1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 16185-1-AP

    IP experiment of mouse heart using 16185-1-AP

    IP result of anti-ING1-specific (IP:16185-1-AP, 2ug; Detection:16185-1-AP 1:300) with mouse heart tissue lysate 4000ug.

ING1-specific Polyclonal antibody

ING1-specific Polyclonal Antibody for WB, IP, ELISA
Cat No. 16185-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, ELISA

别名

ING1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue
Positive IP detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16185-1-AP targets ING1-specific in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
文献引用反应性rat'
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inhibitor of growth family, member 1
别名 ING1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a
计算分子量 47 kDa
观测分子量 30 kDa
GenBank蛋白编号NM_198218
基因名称 ING1
Gene ID (NCBI) 3621
RRIDAB_2233908
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UK53
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING1 is the first identified one, interacting with TP53 and is a component of the p53 signaling pathway. Multiple alternatively spliced transcript variants encoding distinct isoforms of ING1 have been reported. This antibody recognizes all 24/33/47kd isoforms of ING1, but will not bind ING2, 3 or 4.

实验方案

Product Specific Protocols
WB protocol for ING1-specific antibody 16185-1-APDownload protocol
IP protocol for ING1-specific antibody 16185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols