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验证数据展示

  • WB analysis of human kidney using 16187-1-AP

    WB analysis of human kidney using 16187-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 16187-1-AP

    IP experiment of mouse kidney using 16187-1-AP

    IP result of anti-ING3-specific (IP:16187-1-AP, 4ug; Detection:16187-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

ING3-specific Polyclonal antibody

ING3-specific Polyclonal Antibody for WB, IP, ELISA
Cat No. 16187-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

Eaf4, ING2, ING3, p47ING3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, mouse heart tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16187-1-AP targets ING3-specific in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inhibitor of growth family, member 3
别名 Eaf4, ING2, ING3, p47ING3
计算分子量 47 kDa
观测分子量 46 kDa
GenBank蛋白编号NM_019071
基因名称 ING3
Gene ID (NCBI) 54556
RRIDAB_2264993
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXR8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.

实验方案

Product Specific Protocols
WB protocol for ING3-specific antibody 16187-1-APDownload protocol
IP protocol for ING3-specific antibody 16187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols