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验证数据展示

  • WB analysis using 67754-1-Ig (same clone as 67754-1-PBS)

    WB analysis using 67754-1-Ig (same clone as 67754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    IHC staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ING4 antibody included
  • IF Staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    IF Staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ING4 antibody (67754-1-Ig, Clone: 1A12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

ING4 Monoclonal antibody, PBS Only

ING4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67754-1-PBS

产品说明书

CloneNo. 1A12A3

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, rabbit, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

ING4, Inhibitor of growth protein 4, my036, p29ING4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ING4 Ready-To-Use IHC Kit. ING4 primary antibody included.

产品信息

67754-1-PBS targets ING4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, pig, rabbit, rat
免疫原 ING4 fusion protein Ag4610 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 inhibitor of growth family, member 4
别名 ING4, Inhibitor of growth protein 4, my036, p29ING4
计算分子量 29 kDa
观测分子量29 kDa
GenBank蛋白编号BC007781
基因名称 ING4
Gene ID (NCBI) 51147
RRIDAB_2918522
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UNL4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion.