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ING4 Monoclonal antibody, PBS Only

ING4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12A3

Cat No : 67754-1-PBS

Print datasheet

Synonyms

ING4, Inhibitor of growth protein 4, my036, p29ING4

验证数据展示

  • WB analysis using 67754-1-Ig (same clone as 67754-1-PBS)

    WB analysis using 67754-1-Ig (same clone as 67754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67754-1-Ig (ING4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    IHC staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67754-1-Ig (ING4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ING4 antibody included
  • IF Staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    IF Staining of human colon cancer using 67754-1-Ig (same clone as 67754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ING4 antibody (67754-1-Ig, Clone: 1A12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67754-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ING4 Ready-To-Use IHC Kit. ING4 primary antibody included.

产品信息

67754-1-PBS targets ING4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, rabbit, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, pig, rabbit, rat
Immunogen ING4 fusion protein Ag4610 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name inhibitor of growth family, member 4
Synonyms ING4, Inhibitor of growth protein 4, my036, p29ING4
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC007781
Gene Symbol ING4
Gene ID (NCBI) 51147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNL4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion.