• Featured Product
  • KD/KO Validated

ING5 Polyclonal antibody

ING5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10665-1-AP

Print datasheet

Synonyms

ING5, Inhibitor of growth protein 5, p28ING5



经过测试的应用

Positive WB detected inJurkat cells, PC-3 cells, mouse kidney tissue, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ING5 Ready-To-Use IHC Kit. ING5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10665-1-AP targets ING5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ING5 fusion protein Ag1059 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inhibitor of growth family, member 5
Synonyms ING5, Inhibitor of growth protein 5, p28ING5
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC005370
Gene Symbol ING5
Gene ID (NCBI) 84289
RRIDAB_2280522
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)

实验方案

Product Specific Protocols
WB protocol for ING5 antibody 10665-1-APDownload protocol
IHC protocol for ING5 antibody 10665-1-APDownload protocol
IP protocol for ING5 antibody 10665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Cell Res

LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration.

Authors - Yahan Wu
  • KD Validated
mouseWB

EMBO Rep

SCFJFK is functionally linked to obesity and metabolic syndrome.

Authors - Lin He
humanWB

Oncotarget

The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer.

Authors - Yang Li
humanWB

Oncotarget

ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer.

Authors - Feng Zhang
humanIF

Oncotarget

ING5 suppresses proliferation, apoptosis, migration and invasion, and induces autophagy and differentiation of gastric cancer cells: a good marker for carcinogenesis and subsequent progression.

Authors - Wen-feng Gou
  • KD Validated
humanWB,IHC

Oncotarget

Upregulated in Hepatitis B virus-associated hepatocellular carcinoma cells, miR-331-3p promotes proliferation of hepatocellular carcinoma cells by targeting ING5.

Authors - Yiyi Cao
  • KD Validated