验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, PC-3 cells, mouse kidney tissue, HEK-293 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10665-1-AP targets ING5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | ING5 fusion protein Ag1059 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | inhibitor of growth family, member 5 |
别名 | ING5, Inhibitor of growth protein 5, p28ING5 |
计算分子量 | 28 kDa |
观测分子量 | 28-32 kDa |
GenBank蛋白编号 | BC005370 |
基因名称 | ING5 |
Gene ID (NCBI) | 84289 |
RRID | AB_2280522 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8WYH8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223)
实验方案
Product Specific Protocols | |
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WB protocol for ING5 antibody 10665-1-AP | Download protocol |
IHC protocol for ING5 antibody 10665-1-AP | Download protocol |
IP protocol for ING5 antibody 10665-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Res LRP6 downregulation promotes cardiomyocyte proliferation and heart regeneration.
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Oncotarget The miR-193a-3p-regulated ING5 gene activates the DNA damage response pathway and inhibits multi-chemoresistance in bladder cancer. | ||
Oncotarget ING5 inhibits cancer aggressiveness via preventing EMT and is a potential prognostic biomarker for lung cancer. | ||
Oncotarget ING5 suppresses proliferation, apoptosis, migration and invasion, and induces autophagy and differentiation of gastric cancer cells: a good marker for carcinogenesis and subsequent progression.
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Oncotarget Upregulated in Hepatitis B virus-associated hepatocellular carcinoma cells, miR-331-3p promotes proliferation of hepatocellular carcinoma cells by targeting ING5.
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